Vitamin B₆ cofactor-directed fluorescent “turn-on” detection of alkaline-phosphatase activity using bovine serum albumin-functionalized Mn–ZnS quantum dots

A fluorescent turn-on probe for the detection of alkaline phosphatase activity in living cells.

Fluorescent probe 1, showing a high fluorescence turn-on signal ratio, enables the real-time imaging of endogenous alkaline phosphatase (ALP) activity in living cells, and the fast and quantitative analysis of enzyme activity at the single-cell level.

Depression of serum alkaline phosphatase activity by human serum albumin.

The daily intravenous administration of 50 gm. of concentrated saltpoor human serum albumin’ produces a rise in serum albumin level and a decline in urinary calcium and phosphorus excretion (1). These latter changes have been interpreted as evidence for an albumin-induced stimulation of osteogenesis (2). The consistent finding of a concomitant depression of serum alkaline phosphatase activity w...

Spectroscopic investigation of interaction between bovine serum albumin and amine-functionalized silicon quantum dots.

We have investigated the dynamics and mechanistic details of the interaction between bovine serum albumin (BSA) and allylamine-capped silicon quantum dots (Si QDs) by means of fluorescence spectroscopy, circular dichroism (CD), and FTIR spectroscopy. The intrinsic fluorescence of BSA gets quenched in the presence of Si QDs due to ground-state complex formation. The binding stoichiometry and var...

Fluorescence detection of alkaline phosphatase activity with β-cyclodextrin-modified quantum dots.

An alkaline phosphatase activity detection system was constructed based on the different quenching effect of the enzyme substrate and product on the β-CD-functionalized CdTe QDs.

Skeletal alkaline phosphatase activity in serum.